Measure before comparing
A scale bar ties pixels to a measured distance. Magnification alone changes with display size, so use calibrated scale information when assessing feature dimensions.
Microscopy images invite discovery. Their meaning comes from the specimen, the signal, the scale, and a trustworthy source.
Explore the collectionCropped confocal image of a cell on a fiber scaffold · NIST · Source and credit ↗ · Not Phoenix College data
Acquired micrographs record an instrument’s response to a real specimen. Illustrations help explain an idea without carrying measurement data.
Each image below is labeled. Reference micrographs link to their original publishers and state scale information when the source provides it. Illustrations have no implied scale or specimen identity. None is represented as a Phoenix College micrograph.
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Showing all 13 images.
Surface texture recorded at 671×; the source image carries instrument settings and a 50 μm scale bar.
CDC / Janice Haney Carr; Betsy Crane also listed. Public domain. Source and credit ↗Fluorescence-inspired artwork illustrates how separate labels can make different cellular structures visible.
Generated for this site. No acquired specimen, instrument metadata, or scale is implied.A diatom-inspired form conveys the spatial patterns that electron imaging can reveal.
Generated for this site. No acquired specimen, instrument metadata, or scale is implied.Olivine crystals, plagioclase groundmass, and spinel inclusions appear under crossed polarizers. Embedded scale bar: 200 μm.
Brandi Lawler, University of Wyoming / USGS. Public domain. Source and credit ↗Interlocking, colored grains suggest the optical variety visible in birefringent materials.
Generated for this site. No acquired specimen, mineral identification, or scale is implied.Brighter areas indicate greater height, from 0 to 10 nm. The field is roughly 1,700 nm across; color encodes measured height.
MacCuspie / NIST. Source and credit ↗CDC’s 1959 image shows a mixed culture of bacilli and cocci under ultraviolet illumination. The source does not give a scale bar; this is not a confocal image.
CDC Public Health Image Library, image 12118. Public domain. Source and credit ↗Digitally colorized TEM of virions from a 2009 pandemic influenza A(H1N1) isolate. CDC notes varied shapes and surface spikes. No scale is provided on the source page.
CDC / C. S. Goldsmith and A. Balish. Public domain; CDC is the free source. Source and credit ↗A human bone marrow stromal cell on a polymer scaffold. Green marks fibers, red marks actin, and blue marks the nucleus; NIST reports an image cube of 246 × 246 × 29 µm.
National Institute of Standards and Technology (NIST). Source and credit ↗NIST’s false-color SEM shows gold nanoparticle reference standards at 250,000×. The publisher gives no physical scale bar here; display magnification alone is not a measurement.
Andras Vladar / NIST. Source and credit ↗Red blood cells are enmeshed in a fibrin matrix on a catheter surface. CDC records 5,716× magnification; the full image retains a 5 µm scale bar and instrument settings.
CDC / Janice Haney Carr. Public domain; CDC is the free source. Source and credit ↗NIST’s color-added TEM identifies a silicon nanowire within a thin dielectric stack. The original annotations and 40 nm scale bar remain in the image.
Bonevich / NIST. Source and credit ↗Crossed polarizers reveal minerals in a Mesaba copper-nickel deposit drill-core sample. The full image preserves its mineral labels and 1000 µm scale bar; “B1-95” is the core number.
Ruth Schulte / USGS. Public domain. Source and credit ↗A scale bar ties pixels to a measured distance. Magnification alone changes with display size, so use calibrated scale information when assessing feature dimensions.
SEM, fluorescence, and polarized light each produce contrast through different physical signals. A bright area does not mean the same thing in each method.
In this collection, AFM color encodes height, confocal color marks fluorescent labels, and polarized color depends on optical properties. The H1N1 TEM and gold SEM are digitally colorized; NIST added color and annotations to the silicon nanowire TEM.
For method details and image integrity practices, continue with the advanced field guide ↗.